详细信息:Black-Gold™ II stain is an aurohalophosphate complex which stains specifically for myelin within the central nervous system. Black-Gold II staining provides high resolution, high contrast, a short histochemical processing time, and high reproducibility. Black-Gold II stain is a new and improved version of its predecessor, Black-Gold stain (discontinued). The advantages of Millipore’s Black-Gold II over Black-Gold I stain are that it is more readily soluble, can be utilized at a higher concentration, produces a more uniform and consistent staining, takes less time to stain, and does not require post-staining intensification steps.
The use of Black-Gold II stain is tailored to studies using formalin-fixed, non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while the individual myelinated fibers appear black. This novel tracer can be used to localize both normal and pathological myelin. Black-Gold II stain can demonstrate and characterize specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, methamphetamine, acrylamide, domoic acid, 3-nitropropionic acid, Fluoro-Gold™ tracer and isoniazid.
Black Gold II Purity: No detectable amount of uncomplexed gold was found.
Black Gold II Illumination: Bright field or dark field.
Black Gold II Solubility: Freely soluble in water, saline, or dilute acids
